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differentially expression analysis using raw read counts

I have raw read count from RNA-seq(meaning I aligned fastq files and got raw read counts using HTSeq). is there any way to do differentially expression analysis to identify DEGs? thanks

rna-seq

Hi Ashkan,

Please have a look here and here.

~ Best

2 answers

Have a look at DESeq2, edgeR, limma-voom and perhaps this workflow from Bioconductor http://www.bioconductor.org/help/workflows/rnaseqGene/

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