differentially expression analysis using raw read counts
I have raw read count from RNA-seq(meaning I aligned fastq files and got raw read counts using HTSeq). is there any way to do differentially expression analysis to identify DEGs? thanks
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Have a look at DESeq2, edgeR, limma-voom and perhaps this workflow from Bioconductor http://www.bioconductor.org/help/workflows/rnaseqGene/
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Here is tutorial using DESEQ2
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Hi Ashkan,
Please have a look here and here.
~ Best
this tutorial is very useful
C: DE analysis by edgeR