Dear Biostars,
I am working on to get all human embryonic stem cell (hESC) experiments ChIP-seq data from available databases like GEO and SRA databases. As a newbie I dont know how and where to start to complete this task. Can someone help in this regard. Thank you.
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written
by
ramukavuri1 •
2 answers
Term name ES-I3 (I guess these cells are undifferentiated)
Term ID EFO:0007076
Summary ES-I3 stem cell female embryo
Description The I3 stem cell line was derived from donated blastocysts by removal of the trophoblast layer and further culturing of the intact inner cell mass on mitotically inactivated mouse embryonic fibroblast (MEF) feeder cells. Following injection into SCID mice, the I3 line formed teratomas that contained tissues from all three embryonic germ layers. This line exhibits a normal XX karyotype. These cells were provided by Alex Meissner and maintained in mTeSR medium.
https://www.encodeproject.org/reference-epigenomes/ENCSR747ULU/
http://www.ncbi.nlm.nih.gov/geo/roadmap/epigenomics/?view=samples&sample=ES-I3%20cell%20line
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