WGS .fsa_nt file to .fastq.gz
I would like to convert the .fsa_nt file to a fastq.gz file in order to use it in Velvet.
Any help appreciated. Thanks!
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harold.smith.tarheel
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nidhiv •
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nidhiv •
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If this is a multi-fasta format sequence file then you can use reformat.sh from BBMap suite to convert it to fastq (albeit with fake q-scores, 35 in example below) like so:
reformat.sh in=test.fa out=fake.fq.gz qfake=35
Note: SourceForge site appears to be offline at this time. Once it returns you can download BBMap.
See this earlier post: Convert FASTA to FASTQ
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