This is a test version of Biostars. For the public version, visit https://www.biostars.org.
Can I show IGV only with reads of phred score higher than a threshold?

I would like to show reads that has phred score higher than certain threshold? Probably I should filter the bam file with samtools first, but is there any way to filter within IGV?

igv

1 answer

If you want to visually look into reads with certain mapping quality.

Go to preference -> Alignments -> mapping quality threshold

http://www.broadinstitute.org/software/igv/sites/cancerinformatics.org.igv/files/preferences_alignments.png

No, I want them to be out of the figure, those with low phred score.

You mean visually on the browser, the reads with less mapping quality should not be visible, Is'nt it? If it is so, the above solution will work.

Log in to answer this question.