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Alternative splicing using rMATS

I would like to study alternative splicing profile of several patients.I need a tool that takes bam file as input and creates output with number of splice variants for each gene ( ex. exon skipping, intron retention, mutually exclusive, alternative 3' acceptor site...). I tried MISO but it keeps giving me error and I was not able to troubleshoot. I found rMATS easy to run but it compares two samples at a time.

Any suggestion would be appreciated!!!

rna-seq alternative splicing mats

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