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Total Read Count

Hey I am working with the ngs data of mirnas. How do we find the total read count of mirnas ??

rna-seq next-gen

This is a very simple-sounding question, but unless you know exactly what a read is, it's a difficult one to answer. I hope you don't mind that I looked at some previous questions you asked, and from what I read I understand that you're trying to do some bioinformatics without much experience or help from inside your lab. This is a really unfortunately situation.

Questions like "how do i run X" have only two outcomes - you run X, or you fail to run X.

Questions like "how many reads do I have" however, have multiple possible answers. You really need to know the difference between each answer - especially when working with miRNA. I would seek help from someone in your building who can help you learn the basics quickly :)

1 answer

HI,

I think you can get that information in many ways. Mapping or viewing in FASTQC or generating count table will enable you to identify the total number of counts.

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