Hi my friends I have a huge nunber of prob id codes. and I need their gene names and also their protein. I use Annotation for find the genes name and also uniprot codes seperatly to determine the protein . But some of probs id do not have uniprot codes.please help me to find the protein name of those which do not have uniprot id. thanks
2 answers
see the following post for proteins with id:
How to get a fastA file with amino acid sequences from a list of accession numbers
try to use blast against UniProtKB or any other DB within UniProt If some protein doesn't have uniprot ID
I’ve checked this approach for some fragment of Aspergillus protein:
APGHRDFIKNMITGTSQADCAILIIASGTGEFEAGISKDGQTREHALLAFTLGVRQLIVALNKMDTCKWS EDRYNEIVKETSNFIKKVGYNPKAVPFVPISGFNGDNMLEPSPNCP
and got this:
http://www.uniprot.org/blast/uniprot/B2016043046C5QFYU9R
I repeated the same for bacterial protein:
gi|85676342|dbj|BAE77592.1| chromosomal replication initiator protein DnaA, DNA-binding transcriptional dual regulator [Escherichia coli str. K-12 substr. W3110] MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPNRFVLDWVRDKYLNNINGLLTSFCGAD APQLRFEVGTKPVTQTPQAAVTSNVAAPAQVAQTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDN FVEGKSNQLARAAARQVADNPGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDM VKALQNNAIEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEINGVED RLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVRELEGALNRVIANANFT GRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVADLLSKRRSRSVARPRQMAMALAKELTNH SLPEIGDAFGGRDHTTVLHACRKIEQLREESHDIKEDFSNLIRTLSS
I got the following with DnaA from E.coli:
Do you have examples of the codes you have? Have you tried UniProt's ID mapping service? http://www.uniprot.org/uploadlists
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