Samview from bam file aligned to cDNA reference
0
0
Entering edit mode
8.0 years ago
bharata1803 ▴ 560

Hello,

So I have a bam file from alignment to cDNA reference from Ensembl Hg38. Suppose transcript ID is T1. The length of the transcript is 15000. I want to extract the reads that aligned to T1 at position between 200-400. I think this is possible for the ordinary bam file which is aligned to human genome reference. I remember just specify chr1:pos1-pos2. How can I extract reads that correspond to a particular transcript ID and base position.

RNA-Seq sam bam • 2.0k views
ADD COMMENT
0
Entering edit mode

if you know the position of T1 on the genome, the process is called "translation" ...

ADD REPLY

Login before adding your answer.

Traffic: 2160 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6