Paper(S) Discussing Issues In Quality Assessment Of Ngs Data
Hi,
I'm wondering if anyone is aware of any good review papers outlining the main issues in quality assessment of NGS data, particularly Illumina RNA-seq data if at all possible.
I've used FastQC for quality assessment, but it isn't published and I'm wondering if there are other papers which back it up.
Thanks!
2 answers
Take a look at the picard CollectRnaSeqMetrics tool. I'm not suggesting that it is the only tool, but it does give some useful statistics and will certainly highlight samples that are outliers after alignment.
• 1 views
•
link
written
by
Sean Davis
You might find this BioStar link of use.
My own suggestion of htSeqTools and HOMER is still for general NGS datasets.
Log in to answer this question.
More posts like this
-
Direct comparison of epitope-tagged vs endogenous (ChIP-seq)
written by jotan
-
Why does this plot of human GC-content have a peak at around 60%
written by willj •
-
Easy way to calculate NQS (Neighborhood Quality Standard) Score?
written by jik664 •
-
Datasets For Which There Is Microarray And Rna-Seq Data On The Same Samples
written by Paul
-
Hapmap Release 27 Or 28 With Imputed Genotypes Available?
written by Paul
-
Where Can I Find A Detailed Explanation/Discussion Of Errors In Next Generation Sequencing
written by Rs1 •
-
Links To Good Next Gen Sequence Analysis Training Courses And Centres
written by Travis
-
Possible Reasons For Intronic Snp Coming Out Top Of Gwas.
written by Paul
-
Next Gen Sequencing Vs Microarray Advanced Applications
written by Travis
-
Sliderii For Deep Coverage
written by Wjeck
