Hello.
I'm traying to run a GO analysis with the R package "goseq" but I have problems finding the GO Terms mappings for Prunus Persica genes. I understand that goseq needs the name of the gene and the corresponding GO term (ej: GO:0000014 AT3G52115). In the GDR FTP repository there's a "Prunus_persica_v2.0.a1_genes2GO.txt" file, but when running in goseq it throws me this error:
pwf=nullp(diff_express, bias.data=lengths, plot.fit=TRUE) Error in "row.names<-.data.frame"("tmp", value = value) : duplicate 'row.names' are not allowed In addition: Warning message: non-unique values when setting 'row.names': ‘-’, ‘Prupe.1G015800’, ‘Prupe.1G023400’, ‘Prupe.1G036900’, ‘Prupe.1G051600’, ‘Prupe.1G065300’, ‘Prupe.1G073300’, ‘Prupe.1G081800’, ‘Prupe.1G083200’, ‘Prupe.1G088300’, ‘Prupe.1G095400’, ‘Prupe.1G103000’, ‘Prupe.1G108400’, ‘Prupe.1G120600’, ‘Prupe.1G120800’, ‘Prupe.1G124400’, ‘Prupe.1G131400’, ‘Prupe.1G163700’, ‘Prupe.1G166400’, ‘Prupe.1G166700’, ‘Prupe.1G168000’, ‘Prupe.1G174800’, ‘Prupe.1G185400’, ‘Prupe.1G188100’, ‘Prupe.1G189000’, ‘Prupe.1G193700’, ‘Prupe.1G206400’, ‘Prupe.1G217600’, ‘Prupe.1G218900’, ‘Prupe.1G229000’, ‘Prupe.1G234600’, ‘Prupe.1G236400’, ‘Prupe.1G240700’, ‘Prupe.1G243400’, ‘Prupe.1G244900’, ‘Prupe.1G276000’, ‘Prupe.1G280000’, ‘Prupe.1G285700’, ‘Prupe.1G289900’, ‘Prupe.1G314100’, ‘Prupe.1G332600’, ‘Prupe.1G334500’, ‘Prupe.1G342900’, ‘Prupe.1G350900’, � [... truncated]
I load the data by running this command: cat_map=read.table("./Prunus_persica/GO/Prunus_persica_v2.0.a1_genes2GO.txt", head=F, sep="\t")
1 answer
If you have a gene list and want to find pathways associated with your set of genes, then try GeneSCF V1.1 enrichment tool.
Follow the documentation provided in the tool.
GeneSCF v1.1 enrichment tool now supports all organisms from KEGG.
http://genescf.kandurilab.org/
Example input gene_list format
PRUPE_ppa001641mg
PRUPE_ppa002424mg
PRUPE_ppa002490mg
PRUPE_ppa002592mg
PRUPE_ppa002825mg
PRUPE_ppa002932mg
PRUPE_ppa003086mg
PRUPE_ppa003096mg
PRUPE_ppa003108mg
PRUPE_ppa003316mg
PRUPE_ppa003354mg
PRUPE_ppa003382mg
PRUPE_ppa003458mg
PRUPE_ppa003487mg
PRUPE_ppa003512mg
PRUPE_ppa003635mg
PRUPE_ppa003647mg
PRUPE_ppa003847mg
PRUPE_ppa003869mg
PRUPE_ppa003986mg
PRUPE_ppa003994mg
PRUPE_ppa004007mg
PRUPE_ppa004036mg
PRUPE_ppa004086mg
PRUPE_ppa004145mg
PRUPE_ppa004214mg
PRUPE_ppa004434mg
PRUPE_ppa004441mg
PRUPE_ppa004461mg
PRUPE_ppa004471mg
PRUPE_ppa004610mg
PRUPE_ppa004622mg
Log in to answer this question.