microRNA seq vs RNA seq
I have 1 RNA seq and 1 microRNA seq.
As to analysis (QC and mapping), can we use the same softwares or different?
Should I download microRNA genomes for mapping?
Thanks~~
rna-seq
• 2,654 views
•
link
written
by
jolin0701-dy
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
RNA-seq normalization methods between samples
written by chansik •I'm a beginner in RNA-seq. I'm trying to learn RNA-seq analysis with a practical and simple analysis with public RNA-seq data. I downloaded 9 RNA-seq …
-
Analysis of RNA-seq data of Ion Torrent
written by Wang •Hi, everyone. I'm a newbie to sequence data analysis. Yesterday I came across a sequence data created by Ion Torrent form GEO(GSEGSE89972).I plan to analysis …
-
ChIP-Seq Analysis allele specific way?
written by Susmita MandalHello everyone, I am about to do ChIP-Seq analysis allele specific way. I have not done ChiP-Seq analysis practically, but I have some idea about …
-
paired-end, single-end, polyA tail, 500bp, and removal of RNA genes
written by moxuFor DEG analysis using RNA-seq, we typically remove pseudogenes, microRNA genes, and RNA genes such as LINC RNA, SCARNA, SNOR, etc., the reason being that …
-
how to take the subset of fastq reads in one specific chromosome
written by jolin0701-dyI have a paired-ends RNA-seq fastq reads and it is too large. I'd like to create a subset of reads only containing the reads mapping …
-
how to keep transcript ids
written by jolin0701-dyI used tophat-cufflinks pipeline for RNA-seq analysis. In the output file, there is only temporarily test ids such as XLOC_000001. Since I use GRCh38 annotation …
-
The pipeline for analysis ChIP seq and RIP seq
written by jolin0701-dyCan I use the same pipeline for ChIP seq and RIP seq? fasts-tools to do quality control bowtie or tophat to do mapping macs to …
-
how to prepare a contaminant list for Bowtie2
written by jolin0701-dyI'd like to prepare a contaminant list to filter contaminant reads mapped to mitochondrial, ribosomal, actin RNA or phi X genomes28 bu Bowtie2. Where can …
-
Do I need to remove adapter if FastQC showed no adapter content.
written by jolin0701-dyI am new to RNA seq analysis. I ran FastQC and results showed there is no adapter content. But there are still some overrepresented sequences. …
-
Using Qubit to quantify microRNAs for TruSeq in small RNA sequencing
written by CandiceChuDVMHi all, For small RNA sequencing, we are requested to submit either **(1) 1 ug of total RNA** or **(2) 10-15 ng of microRNA** Does …
What do you think? How are they different (in terms of characteristics)?
The length is different..
How would you account for that in your QC (and subsequent mapping)? Will you need to trim and considering the different length would your strategy be different for the two sets? Would you map to the same database (or use something specialized in case of microRNA)?
I see, we should use different software…… thanks for your questions….. I'm a new beginner of rna seq and learning by myself now.. :P