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The pipeline for analysis ChIP seq and RIP seq

Can I use the same pipeline for ChIP seq and RIP seq?

fasts-tools to do quality control

bowtie or tophat to do mapping

macs to do peaking calling.

I thinks they can be analyzed in the same pipeline but for the downstream analysis is different..

Thanks...

chip-seq

1 answer

If you look at this thread you can get a clear idea of how the analysis evolved over the years and that as of now it might not be a very good ploy to use ChIPSeq peak calling for the same.

It depends what you are interested to find. I have no idea of RIP-Seq but this is what I found might be useful. So it is usually dependent on the IPed protein. Having said that take a look at this thread and this link (gives quite a number of tools for RIPseq analysis)

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