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fastq to full genome

Hai

I want to convert fastq (both forward and backward) of a bacterium and corresponding reference sequence . I want to convert that into full genome. which tool is useful to do this?

genome ngs

Hi, you might not get the desired answer because your question is ambiguous, please explain which data you have. If you have fastq reads and a reference sequence, what is the difference of your reference to a 'full genome'? Do you want to assemble the reads?

i want to assemble my reads into full length.

1 answer

actually u can easily convert fastq file to fasta with generation of qual file

awk 'BEGIN{P=1}{if(P==1||P==2){gsub(/^[@]/,">");print}; if(P==4)P=0; P++}' input.fastq > output.fasta

may be he wants to assemble reads to genome (contigs/ scaffolds) based on ref. genome

yes i want to assemble reads

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