Searching for primers (and its rev compl.) in PACMAN
Just curious if this is doable with a simple terminal command line.
I have a file of primers (each primer is /n - new line - delimited). I also have a text file (I guess, also fasta format is easily convertible) of the entire pacman (BAC) sequence.
I would like to feed my primer file and the reverse complement file to see if there is any matches in the BAC file. If there is, I would like to have it return which primer sequence matched.
Command line or a tool - I have no preference. Thank you very much!
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Biomonika (Noolean)
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umn_bist •
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umn_bist •
1 answer
Try primersearch from EBOSS.
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Biomonika (Noolean)
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