Dear all,

I would like to use cutadapt to trim the primers from my sequencing run. I was just wondering when I give the sequence of my primer to cutadapt, do I also have to give the complementary sequence as well?

for example let's say my primer is: AATC

Then is this command "cutadapt -g AATC input.fa output.fa" sufficient to remove the primer sequence or I also have to run the following command:

cutadapt -g TTAG input.fa output.fa

Thanks in advance