Failed to align paired reads
I used Sprades to assemble bacterial genome that sequence from Mi-Illuminar. It's failed to align paired reads. I try to merge them and it is the same. Does anyone have any idea about this?
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Chris Miller
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dogdif •
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Sorry, but you're going to have to provide much more information to get a useful response. You're welcome to edit the question to do so. If not, we're likely to close this question up as unanswerable.
You used SPAdes to create an assembly but now you are unable to align the original reads to this assembly. Was the SPAdes run setup correctly? How many contigs did you end up with? What NGS aligner have you tried?