Why do we required de novo sequencing if almost all the organisms are being sequenced? Why Don't we only focus on reference based sequencing?

Hi all, For doing an RNA-seq analysis, I used STAR for mapping reads to the genome followed by Stringtie for genome-guided assembly. I downloaded the …

Hi all, I'm trying to use cufflinks and related programs (cuffmerge and cuffdiff) to find differentially expressed genes and some splicing events between two conditions …

What is the difference between Genome fraction and Genome coverage while aligning contigs on reference genome in de novo genome assembly?

What is .seq and .reads format? Can i convert my illumina fastq file into .seq or .reads format?

Is there any way to forbid transcript assembly and only use the annotation file in cuffdiff/cufflinks? It seems that the software automatically do the assembly …

Bowtie2 VS Tophat What's the difference between them? and why Tophat required Bowtie2 to be already installed in your system?

<p>What is N50 in de novo genome assembler &quot;Velvet&quot;? and why we calculate it?</p>

I am using Ion torrent for sequencing. In Torrent Browser Analysis Report read depth always shown as blank after complete sequencing. Why? What I am …

<p>Hi,</p> <p>I am working with cufflinks,cuffdiff for RNA-SEQ data. </p> <p>In cufflinks, If we are not looking for novel isoforms, we need to use GTF …