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I want to check if a disease is heritable and what is the chance that the offspring will have the same disease in near future .

Respected Members,

I want to check if a disease is heritable and what is the chance that the offspring will have the same disease in near future.

Which procedure should I follow (RT-PCR or PCR-RFLP or any other procedure )... I need some protocol

Thanks for Help in advance!

gene
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updated by Ram Ram • written by Being Bioinformatician Being Bioinformatician

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written by Michael Dondrup Michael Dondrup

2 answers

  1. This isn't really a bioinformatics question, it's just about which molecular biology method to use.
  2. The answer depends on the nature of the disease. If it's due to a CNV, then RT-PCR would be useful. If it's due to a SNP or InDel then PCR-RFLP might work, though I'd personally just do sequencing of the whole gene.

This all assumes a simple Mendelian disorder, of course.

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written by Devon Ryan Devon Ryan

If the OP is asking about whether or not it is even heritable then you are starting even earlier than that in the process. Whether it is even a genetic disease. If for some reason the OP thinks it is genetic then the standard would be to see if it is or is similar to other known Mendelian disease. You can start by testing those genes known to cause the same or similar diseases, or go straight to something like Exome sequencing.

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written by DG DG

I want to check if a disease is heritable

As Dan Gaston hints to, you don't need a molecular biology technique for this. Depending on your study system you can e.g. regress the phenotype of the offspring on the average phenotype of the parents. The slope of the regression line is the heritability. Molecular biology techniques then allow you to map the gene(s) underlying the phenotype.

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written by dariober dariober

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