How can I do blast of cDNA library in single command, and stored the top 10 result in single file

I have data from cDNA library. I wanted to do blast the entire library against protein database. Is there any possibiility to do blast the entire library in single run and stored the result information in separate file?

blast sequencing

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updated 3.6 years ago by Ram

• written 11.1 years ago by tcf.hcdg

2 answers

If you use stand-alone blast, it is a simple matter of storing all queries in a single FASTA file to use as the query input and either using the remote option or downloading the relevant database to run BLAST.

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written 11.1 years ago by Ram

blastx -db proteindb -out out.blast < input.fa

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updated 3.6 years ago by Ram

• written 11.1 years ago by Pierre Lindenbaum

I like how you cut to the chase

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written 11.1 years ago by Ram

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