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BLAST database index file

When attempting to run protein BLAST locally against the nr database (downloaded from https://www.ncbi.nlm.nih.gov/public/ ) I get an error stating that I need an index file. Do I need to run makeblastdb on this file before I can use it? Should I download the nr database elsewhere? Thanks!

blast

Thank you. Realized it's a firewall issue not allowing me to access the ftp site which is why I searched for the database elsewhere.

You need to download all nr* files from the database link posted by @gb. Uncompress and store them in one folder.

What you downloaded from the link you posted in original post are just fasta formatted files for nr.

Thank you. I initially tried downloading from the ftp site but it fails to open. I'll try the tool you mentioned.

Please do not add answers unless you're answering the top-level question. Instead, use Add Comment or Add Reply as appropriate. I've moved your post to a comment for now, but please be more careful in the future.

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