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P-value histogram interpretation

How I should interpret a histogram of P-values (coming from multiple test) that is increasing in the left side?

Thanks

rna-seq
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updated by Ram Ram • written by Inbalor • Inbalor

1 answer

That suggests that you have some actual significant findings. Under the null, we expect a roughly even distribution of p-values.

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written by Devon Ryan Devon Ryan

Thanks to reply my question.

I understand that if the proportion of genes with p-values < 0.05 is higher that what you expected is because you have significant results. However, what happened if you have less proportion of genes with p-values < 0.05 that what you expected at random. Why it indicates that your results are significant. Sorry, could you explain me better. Thanks again!

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written by Inbalor • Inbalor

Perhaps you're plotting things backward from the rest of us, though. If you're seeing significantly fewer significant p-values than expected even under the null then there's often a confounder that's been omitted from your design (at least in my experience).

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written by Devon Ryan Devon Ryan

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