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This is more appropriate for SEQanswers, so I'll close the post. BTW, you generally want to run a gel after doing a PCR with the primers when things like this occur. If you only get one band with the gel, then try using sequencing primers internal to the amplified region (this is generally a good idea anyway).
Hello Elnaaz!
We believe that this post does not fit the main topic of this site.
Not actually bioinformatics related, try SEQanswers.
For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.
If you disagree please tell us why in a reply below, we'll be happy to talk about it.
Cheers!