sequencing troubleshootes with sanger sequencing
Hi
I did my sequencing by sanger and I used for 1 sample two times sequencing to make sure by 2 primes which are amplifying same parts as a forward and reverse and I have 2 sequenced which supposed to be same but these 2 sequences are different ,,,,what is the problem? May it happen by error of sequencing?
It's interesting that with 2 same primers and same samples you get different sequence!
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
How do I calculate read depth of sequencing?
written by Madde •
-
16S rRNA merge forward and reverse primers
written by ddowlin
-
Preparing 16S gene amplicon (metagenomic) sequencing data for submission to ENA
written by KQUB •
-
FASTQ- Reverse reads of paired end sequence worse than forward reads
written by dilawerkh4 •
-
How to combine a run with quality paired sequences and a run with only quality forward reads
written by Yongjie Zhang
-
my sanger sequencing does not work
written by Elnaaz •
-
sanger sequencing problems
written by Elnaaz •
-
Next Generation Sequencing allignment INDIVIDUAL from chromatogram to POOL IN tablet software to f…
written by Elnaaz •
-
Getting skewed ratios of heterozygosity between Ion Torrent and Sanger sequencing.
written by svalpereira •
-
What's the possible causes of strand bias in exome sequencing?
written by wangyi2412
Hello Elnaaz!
We believe that this post does not fit the main topic of this site.
You might be better off on SeqAnswers, as this is not a bioinformatics question.
For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.
If you disagree please tell us why in a reply below, we'll be happy to talk about it.
Cheers!