detect SNPs using DNAseq fastq files which were used to assemble the genome
Hello all,
I find a problem that interest me. The chinese hamster genome was assembled using DNAseq. And when I tried to use the same DNAseq to call SNPs, it detected some SNPs. My question is: the assembly should not change the sequence, so there should be no SNPs if I use the same DNAseq which was used to assemble the genome. Does anyone know why this happend? Thanks.
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