Ok that makes sense, thank you!
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Hi Biostars, is there a command-line way to guess the best outgroup? Command-line tree-builders such as raxml can accept an outgroup as an argument but sometimes it is not something known a priori.
For most software the tree seach uses unrooted trees, so the outgroup you set is of no consequence -- it just roots it at the end (section 10 of the raxml quickstart makes this clear, for instance).
You should just estimate you tree, then use mid-point rooting if it's important that you use rooted trees in down-stream analyses.
Ok that makes sense, thank you!
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I think that one thing that could be helpful, for example, is that I have a pairwise distance file with three tabs:
And I have a perl script to total the distance although probably not optimized
However, I think that because of a sampling bias, it is not giving me the correct outgroup.