Biomarker Identification with Whole Genome Sequence Alignment
Hello, I am attempting to identify a biomarker target to identify a particular bacteria using PCR because the current available gene targets do not discriminate the bacteria I want to identify from closely related strains. How should I go about this. I was thinking of using whole genome sequence alignment software and aligning whole genome sequences for the particular bacteria I want to identify and then aligning this to the genome of the bacteria I don't want to identify, so as to identify possible target regions. Any advice?
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
Confirming Identity of clone in Phylogeny
written by Jaykumar •
-
Best way to design PCR primers to differentiate fungi strains? Should I be targeting structural va…
written by Mikhail •
-
Partial Genome alignment
written by hunterkwalt •
-
How to find conserved regions in a genome
written by Nicolas •
-
Whole genome Pn/Ps selection stats
written by sarah.goldstein •
-
Detect repeats problematic for PCR
written by mschmid
-
How to find strain specific truncated genes?
written by mmart12 •
-
Bacterial identification - PCR primers - unknown target
written by RAZLICAK •
-
Design Pcr Primers To Uniquely Identify A Bacterial Strain
written by Eric Normandeau
-
Estimate The Impact Of Gene Loss In Silico
written by qiyunzhu
Check this out: http://bioinformatics.oxfordjournals.org/content/27/3/334.long. The paper claims that the tool is suited for homologous genomes and provide sequence conservation as output. So you could manually select some of the least-conserved genes.