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questions
7
votes
4
replies
5.4k
views
Which aligner is best suited for circular human mitochondrial genome?
bowtie2
Assembly
Alignment
bwa
updated 21 months ago by
Ram
43k • written 8.4 years ago by
nalandaatmi
▴ 100
0
votes
13
replies
14k
views
Counting number of genes using htseq-count tool.
RNA-Seq
htseq
count
rna
updated 20 months ago by
Ram
43k • written 8.6 years ago by
nalandaatmi
▴ 100
1
vote
3
replies
3.8k
views
Difference in number of reads in tophat and htseq-count outputs
next-gen
RNA-Seq
ChIP-Seq
sequence
alignment
updated 20 months ago by
Ram
43k • written 8.6 years ago by
nalandaatmi
▴ 100
1
vote
4
replies
3.2k
views
Cuffdiff terminated after GffObj::getSpliced() error
cuffmerge
cuffdiff
RNASeq
updated 21 months ago by
Ram
43k • written 8.4 years ago by
nalandaatmi
▴ 100
0
votes
6
replies
5.9k
views
Output files are empty after adapter trimming using trim galore
trimgalore
adapter
trimming
RNASeq
updated 21 months ago by
Ram
43k • written 8.4 years ago by
nalandaatmi
▴ 100
2
votes
6
replies
5.8k
views
output SAM file from STAR aligner looks incomplete
DNAseq
alignment
aligner
STAR
updated 21 months ago by
Ram
43k • written 8.4 years ago by
nalandaatmi
▴ 100
6
votes
15
replies
8.5k
views
Strand specific protocol for bowtie2 alignment
bowtie2
rnaseq
alignment
RNA-Seq
updated 2.5 years ago by
Ram
43k • written 8.5 years ago by
nalandaatmi
▴ 100
1
vote
5
replies
1.9k
views
Observed and expected fastq header count different when merging two fastq files
RNA-Seq
next-gen
fastq
updated 20 months ago by
Ram
43k • written 8.5 years ago by
nalandaatmi
▴ 100
5
votes
5
replies
9.5k
views
Difference in htseq-count values for sorted bam and unsorted bam by name
RNA-Seq
htseq-count
sequencing
SNP
updated 21 months ago by
Ram
43k • written 8.6 years ago by
nalandaatmi
▴ 100
1
vote
2
replies
2.0k
views
Have anyone worked with Genevar tool for eQTLs and mQTLs from sanger
next-gen
variant-calling
SNP
software-error
updated 20 months ago by
Ram
43k • written 8.6 years ago by
nalandaatmi
▴ 100
5
votes
5
replies
3.9k
views
How to interpret the difference among these three options in strandedness from HTSeq-count
RNA-Seq
next-gen
sequence
alignment
updated 20 months ago by
Ram
43k • written 8.6 years ago by
nalandaatmi
▴ 100
11
votes
9
replies
13k
views
I have same samples in multiple lanes. What are all the steps to be taken before downstream analysis?
fastq
alignment
next-gen-sequencing
RNASeq
updated 19 months ago by
Ram
43k • written 8.6 years ago by
nalandaatmi
▴ 100
0
votes
13
replies
2.9k
views
How to do sequencing reads technical analysis and biological quality analysis for RNASeq dataset?
RNA-Seq
next-gen
alignment
updated 19 months ago by
Ram
43k • written 8.6 years ago by
nalandaatmi
▴ 100
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