We have sequenced mitochondrial DNA samples. Initially the reads were mapped against the mitochondrial chromosome alone. There were some false positive hits. Inorder to avoid false positives hits. This time I am planning to map the reads against the whole human genome.
Then I have been asked to take the reads that are failed to map in previous step should be remapped to mitochondrial chromosome. While remapping the reads, they stressed me to consider the fact of circular genome. Which will lead to a flat pileup of the reads across the whole mitochondrial chromosome, instead of the ends show a dropout in reads (pileup looks like inverted U-shape).
As you suggested, I am planning to use bwa-mem aligner. but I don't get the highlighted text. Can you explain if you are aware of it.