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1,062 results • Page
3 of 22
Sort: Rank
Rank
Views
Votes
Replies
1
vote
4
replies
386
views
Limma Analysis Agilent Microarray Data (GPL1708)
Microarray
Limma
Agilent
3 days ago by
hagl
▴ 10
0
votes
5
replies
249
views
RNAseq one control two conditions, shared and exclusive genes
conditions
RNAseq
multiple
Rstudio
updated 51 minutes ago by
Mohamed Abderrahmane
▴ 20 • written 3 days ago by
matteo.levorato
• 0
0
votes
2
replies
151
views
Longitudinal analysis of subpopulations: which approach is better?
differential-expression
DEG
model
3 days ago by
Lluís R.
★ 1.2k
0
votes
1
reply
142
views
How to solve DESeq2 Error in checkFullRank(modelMatrix)?
RNA-Seq
DESeq2
updated 4 days ago by
i.sudbery
19k • written 4 days ago by
DOBI
• 0
0
votes
0
replies
97
views
Comparing peptide sequences with MS/MS peptide data using MaxQuant
Transcriptomics
Mass
Bioinformatics
spectrometry
Proteins
4 days ago by
atharvakarkare14
▴ 30
5
votes
16
replies
692
views
How many reads for WGS Sequencing?
WGS
Bacterial-Genomics
4 days ago by
Ruqaiya
• 0
0
votes
2
replies
197
views
What should I consider as FASTA for dataset?
PDB
FASTA
3 days ago by
Nafi
• 0
38
votes
24
replies
1.9k
views
10 follow
NGS forensics: how to know if data is fabricated
fastq
STAR
NGS
Illumina
updated 2 days ago by
i.sudbery
19k • written 5 days ago by
noodle
▴ 580
0
votes
0
replies
89
views
Differential accessibility using DiffBinf
diffbind
4 days ago by
Shloka
• 0
0
votes
0
replies
93
views
vg call vs vg surject
vg
variation
graphs
updated 3 days ago by
GenoMax
142k • written 4 days ago by
aliraza3119
• 0
0
votes
1
reply
131
views
Can I merge Hi-C fastq files from different lanes?
GenomeAssembly
BWA-MEM2
Hi-C
updated 3 days ago by
GenoMax
142k • written 4 days ago by
Winter
• 0
2
votes
7
replies
1.3k
views
6 follow
Differential Expression using Isoseq-supplemented reference transcriptome
RNA-Seq
Salmon
Isoseq
updated 4 days ago by
Gordon Smyth
★ 7.1k • written 8 months ago by
Calum
▴ 10
0
votes
1
reply
206
views
Finding batch and outlayers
Pca
updated 3 days ago by
christopher medway
▴ 450 • written 4 days ago by
Tigran
• 0
2
votes
2
replies
193
views
PDB related issue
rcsb
pdb
updated 4 days ago by
noodle
▴ 580 • written 4 days ago by
Nafi
• 0
0
votes
0
replies
109
views
News:
Online course: Genome Annotation
Genome-Assembly
Genome-Annotation
3 days ago by
carlopecoraro2
★ 2.5k
0
votes
8
replies
425
views
What does it mean single base resolution in sequencing?
SNP
sequencing
updated 2 days ago by
LauferVA
4.2k • written 4 days ago by
jinyu
▴ 10
0
votes
9
replies
2.5k
views
6 follow
Cannot process all the reads in a fast5 file?
metagenome
base-calling
fastq
nanopore
updated 4 days ago by
Ram
43k • written 8 months ago by
Gio
• 0
2
votes
5
replies
450
views
Fetch table from clinvar database according to a list of rsid
python
clinvar
perl
4 days ago by
ashaneev07
▴ 20
0
votes
1
reply
168
views
Downloading full alignments from Pfam
pfam
updated 4 days ago by
GenoMax
142k • written 4 days ago by
bef1
• 0
0
votes
1
reply
747
views
Can I run cellassign on samples independently if there is batch effect present?
RNA-Seq
R
cellassign
batch-effect
updated 4 days ago by
Francesco
▴ 10 • written 3.3 years ago by
gt
▴ 30
3
votes
2
replies
290
views
ChIP-seq datasets: input samples omitted?
ChIP-seq
Normalization
NGS
updated 3 days ago by
i.sudbery
19k • written 6 days ago by
vanbelj
▴ 40
0
votes
0
replies
120
views
adjusting for confounders in LMER in R
confounders
LMER
R
updated 3 days ago by
dariober
14k • written 5 days ago by
rene.j.erhardt
▴ 20
1
vote
3
replies
296
views
How to assign cell types after integration in scRNA
scRNA-seq
updated 4 days ago by
ATpoint
82k • written 5 days ago by
Francesco
▴ 10
0
votes
0
replies
137
views
STAR total splices (in Log.final) vs collapsed splice junctions (in SJ.out.tab)
STAR
5 days ago by
tnminh89
▴ 10
0
votes
0
replies
141
views
Filter low express genes in microarray data
microarray
5 days ago by
Chris
▴ 260
0
votes
1
reply
330
views
absolute path for symbolic links in Snakefile
Snakemake
updated 5 days ago by
Jesse
▴ 740 • written 13 days ago by
yifangt86
▴ 60
0
votes
2
replies
341
views
Hide positions in alignment with 99% "–" characters to ignore single sequence insertions?
alignment
gaps
updated 5 days ago by
Jesse
▴ 740 • written 8 days ago by
Broccoli
• 0
4
votes
3
replies
360
views
Source other conda environments in a nextflow pipeline when nextflow itself is in a conda environment?
hpc
conda
nextflow
updated 5 days ago by
Arup Ghosh
3.2k • written 6 days ago by
chaco001
▴ 40
1
vote
3
replies
382
views
Help understanding how KEGG Ortholog `K00004 ` has 3 ECs associated with it (EC:1.1.1.4, 1.1.1.-, 1.1.1.303)?
ontology
metagenomics
database
enzymes
genomics
updated 4 days ago by
Mensur Dlakic
★ 27k • written 6 days ago by
O.rka
▴ 710
0
votes
2
replies
253
views
Correct way to compare multiple treaments between RNA-Seq samples using edgeR?
RNA-Seq
edgeR
5 days ago by
Guille
• 0
4
votes
2
replies
298
views
How to use limma to find differentially expressed genes in response to a continuous variable
limma
voom
R
3 days ago by
pairedttest
▴ 10
3
votes
6
replies
2.0k
views
7 follow
DNA methylation preprocessing
SWAN
DNA
methylation
Funnorm
updated 6 days ago by
Ram
43k • written 2.4 years ago by
SYOSY
▴ 10
0
votes
0
replies
565
views
Correlation between cell type prediction scores and individual gene expression in spatial transcriptomic datasets
single-cell
Spatial-Transcriptomics
6 days ago by
biocellbio
• 0
4
votes
8
replies
647
views
Create a new bed file with all pairwise combinations between two other bed files, based on bp distance
SNPs
BED
eqtl
bedtools
updated 6 days ago by
Alex Reynolds
35k • written 7 days ago by
J
▴ 10
4
votes
7
replies
684
views
7 follow
Heatmap and rna-seq
RNA-Seq
Heatmap
updated 6 days ago by
dsull
★ 5.9k • written 10 days ago by
qudrat.nii
▴ 10
19
votes
11
replies
14k
views
10 follow
How Can We Find The Info For 3'Utr And 5'Utr In Gencode Gtf File?
utr
updated 6 days ago by
cmdcolin
★ 3.8k • written 10.8 years ago by
J.F.Jiang
▴ 920
3
votes
2
replies
330
views
what is the purpose of indexing the reference genome (Kallisto)
indexing
Kallisto
updated 6 days ago by
dsull
★ 5.9k • written 7 days ago by
Aaliya
▴ 10
3
votes
0
replies
203
views
News:
Webinar “Learning Single-cell Atlases” with Dr. Fabian Theis, author of Scanpy on May 8
Scanpy
Generative-AI
scRNA
Machine-Learning
updated 6 days ago by
Ram
43k • written 6 days ago by
Claire Watson
▴ 60
0
votes
2
replies
281
views
Rare Disease Variant Pathway Analysis
Pathway-analysis
updated 6 days ago by
Ram
43k • written 7 days ago by
The_PyPanda
▴ 10
0
votes
0
replies
204
views
Phasing VCF Files and Analyzing Reads with Multiple Variants
haplotypes
vcf
phasing
6 days ago by
HarperReed
• 0
3
votes
3
replies
303
views
genome assembly records not present in assembly_summary.txt
ncbi
bacteria
assembly
updated 6 days ago by
GenoMax
142k • written 7 days ago by
sapuizait
▴ 10
1
vote
0
replies
158
views
Simulation of label-free bottom-up proteomics expression dataset
label-free
bottom-up
lc-ms
proteomics
7 days ago by
KABILAN
▴ 70
0
votes
0
replies
176
views
Running Phylogenetic Analysis With NCBI Genome
population-genetics
phylogenetic
updated 6 days ago by
Ram
43k • written 7 days ago by
SineWave
• 0
0
votes
0
replies
172
views
RNA-seq: full length gene
RNA-seq
updated 6 days ago by
Ram
43k • written 7 days ago by
Nargis
• 0
1
vote
7
replies
490
views
gvcf joint calling
WES
GATK
VCF
gVCF
5 days ago by
zihanss
• 0
0
votes
0
replies
154
views
Haplotype Phased Assembly Contigs to Chromosome Annotations
Assembly
phased
Haplotype
Annotation
7 days ago by
turcoa1
• 0
3
votes
3
replies
303
views
Sequence read length shorter than flow cell specification
illumina
NGS
sequencing
updated 7 days ago by
swbarnes2
14k • written 7 days ago by
M
• 0
0
votes
0
replies
165
views
Designing single-stable RNA molecules
structure
RNA
7 days ago by
Edna
• 0
0
votes
0
replies
175
views
How to visualize/predict the final transcript from Delly output?
WGS
DELLY
7 days ago by
simplitia
▴ 130
1
vote
1
reply
226
views
Normalize scRNAseq data to housekeeping genes to compare several datasets
RNA-sequencing
housekeeping
Single-cell
normalization
updated 7 days ago by
ATpoint
82k • written 7 days ago by
AaronJaime
• 0
1,062 results • Page
3 of 22
Recent Votes
How to Calulate Allele Frequency from a VCF File?
Answer: allele frequency in VCF
Answer: Two references 1. genome 2. plasmid for bowtie2
A: Iterating through paired-end reads in samtools / pysam
Answer: Where Can I Download Some Bam Files?
Download SAM/BAM files from SRA takes ages!!!
Comment: Bedtools merge minimum overlap?
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Answer: What purposes can TPM values be used for?
by
b.contreras.moreira
▴ 180
In case it helps: https://haroldpimentel.wordpress.com/2014/05/08/what-the-fpkm-a-review-rna-seq-expression-units
Comment: What analysis suitable to identify similarly expressed genes between two samples
by
alifafiq1
• 0
Thank you @AT point. I was planning to use DESeq2 and use the alternate hypothesis (lessAbs) too considering that is the only package that …
Comment: Does comparing two different groups to a common third group introduce bias in th
by
ATpoint
82k
Note that by basic algebra A vs C and B vs C is the same as A vs B, so you might rethink your analysis to answer your question in a much si…
Comment: RNAseq one control two conditions, shared and exclusive genes
by
Mohamed Abderrahmane
▴ 20
Yes, why not? If it works for you like that, bingo!
Answer: Does comparing two different groups to a common third group introduce bias in th
by
christopher medway
▴ 450
You are testing groups A and B to a common baseline (C) - I don't see that there is anything wrong with doing that. It is a fairly common a…
Comment: HOMER on AWS
by
clairechung112
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Hi. I guess it is solved by now, but as I did not find the answer immediately online, here is the answer I posted on a relevant question: h…
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clairechung112
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A late reply, but I just solved exactly the same error upon a request in the team. Please see if it helps in case anyone meets the same err…
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Was the spike-in a commercial product, e.g., from Zymo? Can you provide more information about what cells or DNA was spiked into your samp…
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Note that, in place of `sort-bed - | uniq -`, you can pass the `--unique` option to `sort-bed`. The input is not strictly a BED6 file, but…
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LChart
3.9k
Imputation takes what? A day or two of compute time? Just re-do it.
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Thanks. Really appreciate your help with this. This site is such a great resource. The methods aren't totally clear on how they're gettin…
Comment: How to access TCGA samples that were treated with a specific drug?
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142k
It is possible that these drugs may not have been directly used in TCGA. Authors could have looked for mutations known to be acted on by th…
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Are you thinking about a specific scenario? If so, can you provide more info? I think the basic answer is that, yes, your choice of group C…
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by
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★ 4.9k
Bedtools [intersect][1] allows you to specify the fraction of overlap between two BED (or BAM) files using the F/f/r flags. You could split…
Comment: How to access TCGA samples that were treated with a specific drug?
by
Qroid
▴ 40
Sorry, I should have been more specific. By "that list" I mean what's populated in the Therapeutic Agents tab when no filters are applied. …
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