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4
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0
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118
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Answer:
Answer: PCA plot no distinct cluster. Only p < 0.05 indicates significant DEGs, while ad
8 hours ago by
swbarnes2
14k
0
votes
1
reply
331
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Answer:
Answer: Design for complex RNA-Seq experiment using Deseq2
6 days ago by
swbarnes2
14k
0
votes
1
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795
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Comment:
Comment: DEG analysis with limma and contrast matrix using multiple Parkinson's cohorts i
7 days ago by
swbarnes2
14k
0
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1
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795
views
Comment:
Comment: DEG analysis with limma and contrast matrix using multiple Parkinson's cohorts i
8 days ago by
swbarnes2
14k
0
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1
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596
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Answer:
Answer: In IGV is this a heterogeneous mutation or false call?
13 days ago by
swbarnes2
14k
0
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0
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261
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Comment:
Comment: Differentail gene expression results
15 days ago by
swbarnes2
14k
0
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0
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304
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Comment:
Comment: How to compute TPM normalized values for TCGA miRNA data?
16 days ago by
swbarnes2
14k
0
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0
replies
429
views
Comment:
Comment: Understanding STAR output (Aligned.out.sam file)
16 days ago by
swbarnes2
14k
0
votes
1
reply
379
views
Answer:
Answer: Error in checkFullRank(modelMatrix) : the model matrix is not full rank, so t
18 days ago by
swbarnes2
14k
0
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0
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512
views
Comment:
Comment: Differential Expression Analysis using DESeq2 in R
20 days ago by
swbarnes2
14k
0
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1
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465
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Answer:
Answer: Clarification regarding SAM flags "mate reverse strand" (flag 16/0x10) and "read
20 days ago by
swbarnes2
14k
0
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0
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512
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Comment:
Comment: Differential Expression Analysis using DESeq2 in R
20 days ago by
swbarnes2
14k
0
votes
0
replies
382
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Answer:
Answer: Sequence read length shorter than flow cell specification
28 days ago by
swbarnes2
14k
1
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0
replies
800
views
Comment:
Comment: PCA plot (Suggestions Needed)
29 days ago by
swbarnes2
14k
0
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1
reply
990
views
Comment:
Comment: Adding CB tag to bam file
29 days ago by
swbarnes2
14k
1
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0
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800
views
Comment:
Comment: PCA plot (Suggestions Needed)
29 days ago by
swbarnes2
14k
0
votes
0
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800
views
Comment:
Comment: PCA plot (Suggestions Needed)
29 days ago by
swbarnes2
14k
0
votes
1
reply
638
views
Comment:
Comment: To get p-values for the TPM
29 days ago by
swbarnes2
14k
1
vote
0
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422
views
Comment:
Comment: Should I use unpaired reads from trimmomatic
4 weeks ago by
swbarnes2
14k
0
votes
0
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363
views
Answer:
Answer: from row count to tpm
4 weeks ago by
swbarnes2
14k
0
votes
1
reply
569
views
Comment:
Comment: Marking duplicates using UMIs
4 weeks ago by
swbarnes2
14k
0
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0
replies
568
views
Comment:
Comment: Free/open source 23andme-like analysis
4 weeks ago by
swbarnes2
14k
0
votes
0
replies
1.4k
views
Comment:
Comment: Cellranger count error: Input FASTQ file ended prematurely
4 weeks ago by
swbarnes2
14k
0
votes
1
reply
990
views
Comment:
Comment: Adding CB tag to bam file
4 weeks ago by
swbarnes2
14k
0
votes
0
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829
views
Comment:
Comment: RNA seq differential expression analysis
5 weeks ago by
swbarnes2
14k
0
votes
1
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990
views
Answer:
Answer: Adding CB tag to bam file
5 weeks ago by
swbarnes2
14k
2
votes
1
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753
views
Answer:
Answer: DEG analysis of RNA-seq data across multiple tissues and two conditions
5 weeks ago by
swbarnes2
14k
0
votes
0
replies
650
views
Answer:
Answer: Removing adapter/primer sequence from reads while keeping flanking regions
7 weeks ago by
swbarnes2
14k
0
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1
reply
650
views
Comment:
Comment: Removing adapter/primer sequence from reads while keeping flanking regions
7 weeks ago by
swbarnes2
14k
0
votes
0
replies
467
views
Comment:
Comment: Seeking Help with Unexpected MA Plot Output
7 weeks ago by
swbarnes2
14k
1
vote
0
replies
456
views
Comment:
Comment: Problem generating paired end reads when converting Cell Ranger's BAM result to
7 weeks ago by
swbarnes2
14k
0
votes
0
replies
344
views
Answer:
Answer: Find out shared differentially expressed genes between two cell lines due to kno
9 weeks ago by
swbarnes2
14k
0
votes
0
replies
324
views
Answer:
Answer: Detecting viruses in human rna seq data
9 weeks ago by
swbarnes2
14k
1
vote
1
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445
views
Answer:
Answer: Is it better to pool samples prior to running deseq2, or should the algorithm be
9 weeks ago by
swbarnes2
14k
2
votes
0
replies
635
views
Answer:
Answer: Overriding a color palette in R to emphasize specific data points
10 weeks ago by
swbarnes2
14k
0
votes
0
replies
605
views
Comment:
Comment: RNA_seq
10 weeks ago by
swbarnes2
14k
0
votes
1
reply
605
views
Answer:
Answer: RNA_seq
10 weeks ago by
swbarnes2
14k
1
vote
0
replies
408
views
Answer:
Answer: Is it feasible to merge Illumina RNA-Seq data with varying read lengths?
10 weeks ago by
swbarnes2
14k
2
votes
0
replies
412
views
Answer:
Answer: Re. Batch effect correction in data with a design matrix without full rank
10 weeks ago by
swbarnes2
14k
0
votes
1
reply
579
views
Answer:
Answer: RNA Seq Sample Outlier
10 weeks ago by
swbarnes2
14k
1
vote
1
reply
464
views
Answer:
Answer: STAR alignment setting for RNA-Seq data from mouse background expressing a human
11 weeks ago by
swbarnes2
14k
1
vote
1
reply
549
views
Answer:
Answer: (STAR) What causes a high number of reads that are unmapped because they are too
11 weeks ago by
swbarnes2
14k
2
votes
1
reply
352
views
Answer:
Answer: Single cell rna sequencing analysis using R help
11 weeks ago by
swbarnes2
14k
3
votes
0
replies
469
views
Answer:
Answer: single cell rna seq-how to DEG analyze??(full code & Dimplot added)
11 weeks ago by
swbarnes2
14k
1
vote
0
replies
265
views
Answer:
Answer: biological replicate in STAR command
12 weeks ago by
swbarnes2
14k
1
vote
0
replies
429
views
Answer:
Answer: PCA plot analysis
12 weeks ago by
swbarnes2
14k
0
votes
1
reply
476
views
Answer:
Answer: ENA fastq file shown as single-end but has 2 fastq files: X.fastq.gz and Y.fastq
3 months ago by
swbarnes2
14k
2
votes
0
replies
347
views
Comment:
Comment: Getting less DE genes when incorporating all samples
3 months ago by
swbarnes2
14k
1
vote
0
replies
483
views
Comment:
Comment: Count table statistical analysis
3 months ago by
swbarnes2
14k
1
vote
1
reply
624
views
Comment:
Comment: Trouble Understanding DESeq2 Design Formula ("Model Matrix is not Full Rank")
3 months ago by
swbarnes2
14k
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