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1
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4
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Getting the coding_sequence.fasta from the .gff file from the AUGUSTUS gene prediction.
augustus annotation assembly genome
updated 5 days ago by 8.6k • written 7 days ago by ▴ 30
2
votes
3
replies
338
views
Help with choosing a model species for Augustus for a de novo assembled genome.
augustus annotation assembly genome
updated 17 days ago by ★ 2.0k • written 17 days ago by ▴ 30
0
votes
0
replies
113
views
Successfully identified and Masked rpeats using RM. What Next?
sequence annotation repeatmasker illumina assembly
18 days ago by Vijith ▴ 30
2
votes
3
replies
624
views
Should I use Dfam or a custom repetitve elements library (from PlantRep) as a repeat database; using RepeatMasker in a Linux machine?
genome sequence repeatmasker blast
updated 7 weeks ago by ▴ 200 • written 7 weeks ago by ▴ 30
1
vote
1
reply
289
views
What could be a better K-mer for my WGS assembly task using ABySS, with my current hardware profile.
ABySS genome k-mer assembly
updated 3 months ago by ★ 1.2k • written 3 months ago by ▴ 30
3
votes
7
replies
774
views
"C++ compiler cannot create executables", while installing ABySS 2.0 assembler
assembly illumina abyss btllib paired-end
updated 4 months ago by ★ 1.4k • written 4 months ago by ▴ 30
3
votes
5
replies
1.1k
views
I have a pair of read files where each is a mixed pool of reads from the Linked-read technology and Illumina short-read technology. Any suggestion on…
NGS clc-genomics illumina WGS
updated 7 months ago by 43k • written 7 months ago by ▴ 30
2
votes
3
replies
924
views
I have performed Trimmomatic run for adapter removal. QC report shows drop in the reads and presence of overrepresented sequences. Seeking help!
NGS illumina WGS
updated 7 months ago by 43k • written 7 months ago by ▴ 30
0
votes
0
replies
486
views
Can someone help me to programmatically implement skewed normal distribution fitting on the given data points?
python probability normal distribution
9 months ago by Vijith ▴ 30
23
votes
41
replies
4.5k
views
6 follow
I want to correct the erroneous barcode file, and the Python code that I've written, using Biopython, is very slow. How can I make this process fast…
NGS biopython illumina WGS
updated 12 months ago by 43k • written 13 months ago by ▴ 30
0
votes
0
replies
477
views
In a de novo genome assembly using Tell-Seq, why does Universal sequencing Tell-read pipelines generate "erroeneous barcode" files "?
NGS Tell-seq next sequencing generation
19 months ago by Vijith ▴ 30
4
votes
4
replies
2.3k
views
What are R1, R2, R3, and I1 in a illumina sequencing data, and how can I get more information about them?
illumna NGS generation sequencing next
updated 19 months ago by ▴ 280 • written 19 months ago by ▴ 30
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