Hi, does anyone here know how to reverse the coverage track in IGV? I have separate the reads mapped to the + and -strand and want to display them one upwards and the other downwards, like the picture here:
First, you need to use bedtools to convert bam or bed file into bedgraph format for positive strand and negative strand respectively. Please remember multiply the coverage of negative strand by "-1". Then you can merge these two files together and load it in IGV.