My lab just recently bought CLC Bio workbench. I am getting used to this software, but there is a simple blast analysis that I do not know why it is not giving me the results I need. I downloaded the databases from the broad Institute, I put my sequences in a fasta file, but when I retrieve my results, I can not have the description of the gene under Accession (E-value). Can anybody help me out here?
Update: I contacted both sides (CLC Bio and the Broad Institute) to ask some help. The CLC Bio people basically said that the problem is the Broad Institute database. I am waiting for Broad's response. I know it has to be something very easy, but I do not know what that is.
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Edwin •
I think the right source of information is the provider of that data analysis package. Your purchase includes (or should include) support as well.
Agreed: You should e-mail support@clcbio.com
They should reply pretty quickly - probably within 24 hours
My guess is that CLC typically works differently for a .fasta file versus a BLAST database, where you should be creating that later instead of using the former
Thanks guys. I contacted both sides (CLC Bio and the Broad Institute) to ask some help. The CLC Bio people basically said that the problem is the Broad Institute database. I am waiting for Broad's response. I know it has to be something very easy, but I do not what that is. Thanks
FYI, I'm removing your answer and making it an update of your question.
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When you say "the description of the gene under Accession" - is that something you expect to see in the query sequence (fasta file) or the BLAST hit sequence (the BLAST database)? And if the latter - have you checked that the database was formatted so as to include that information?
Thank you for your response. Yes I checked the database, and there is some information about the gene ID and its characteristics that is included in the database. I went back to check the database from the Broad Institute and the information is there. I contacted the Broad Institute to ask for help. thanks again