**Guides for sequencing** [Designing a sequencing project][1] [Recommended coverage by library preparation application][2] [Comparison of instrument read lengths and read outputs][3] **Guides for preparing your …

Before working with Next-Gen machines or datasets, it's vital to understand how the technology works. Here's the first part of a guide to Next-Generation Sequencing …

Day 1 of the Sequencing, Finishing and Analysis Meeting kicked off with a great series of lectures. We've highlighted some of the talks for those …

Demand for the analysis of next generation sequencing data is growing at a fast rate. To help accommodate this demand, [Genohub.com][1] now allows researchers to …

Standalone program to visualize enrichment patterns of DNA-interacting proteins at functionally important regions based on next-generation sequencing data: http://www.ncbi.nlm.nih.gov/pubmed/24735413 --[Genohub][1] [1]: http://www.genohub.com

<p>Despite the new NextSeq 500, looks like it&#39;s still better to use MiSeq for low diversity samples:</p> <p>http://blog.genohub.com/nextseq-hiseq-or-miseq-for-low-diversity-sequencing/</p> <p>Any other thoughts on mitigating intensity issues …

<p>Interesting paper examines transcriptome reassembly and determines it is sensitive to RNA fragmentation technique. Concludes that Zn mediated fragmentation provides a better transcript ID compared …

AGBT 2014 started off strong with talks on single cell sequencing, next gen diagnostics in cancer medicine and GRCh38. A twitter/blog embargo threw a wrench …

I'd like to hear your experience seeking out a NGS service provider or bioinformatician. What's most painful? Or are you happy? http://blog.genohub.com/matchmaking-for-the-life-scientist/ -- [Genohub][1] [1]: …

<p>Hi, I would be interested in papers comparing different protocols (e.g. different priming methods, different fragmentation methods, etc.) for DNA or RNA seq. I know …