Question

Papers Comparing Different Protocols For Next Gen Seq

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12.2 years ago

Steffi ▴ 580

Hi, I would be interested in papers comparing different protocols (e.g. different priming methods, different fragmentation methods, etc.) for DNA or RNA seq. I know the paper from Levin et al (Comprehehnsive comparative analysis of strand-specific RNA sequencing methods). Does somebody know about other papers?

Thanks, Steffi

next-gen sequencing • 2.3k views

ADD COMMENT • link updated 12.2 years ago by Sravanthi Davuluri ▴ 40 • written 12.2 years ago by Steffi ▴ 580

Ram · Answer 1 · 2012-03-20

Below are two articles, one comparing the methods for fragmentation and other comparing massively parallel sequencers with the currently developing next generation of electrophoresis-based genetic analysis platforms.

1: Knierim E, Lucke B, Schwarz JM, Schuelke M, Seelow D. Systematic comparison of three methods for fragmentation of long-range PCR products for next generation sequencing. PLoS One. 2011;6(11):e28240. Epub 2011 Nov 30. PubMed PMID: 22140562; PubMed Central PMCID: PMC3227650.

2: Hert DG, Fredlake CP, Barron AE. Advantages and limitations of next-generation sequencing technologies: a comparison of electrophoresis and non-electrophoresis methods. Electrophoresis. 2008 Dec;29(23):4618-26. Review. PubMed PMID: 19053153.

For other comparative studies on NGS algorithms, resources, etc., visit the below URL http://www.shodhaka.com/cgi-bin/startbioinfo/inter5.pl?tn=Next%20generation%20sequencing