Good day, I am required to have at least 100X sequencing coverage for SNP primer design
The target organism is a fungus with a genome size of approx. 100Mbp
I am not sure if a 10GB of Illumina data would suffice. So, I am thinking of assessing it using the said sequencing service that produce that data.
The question is if I can do another sequencing of 10GB and pooling them together to achieve the required coverage
Example: PE data 1= 75X PE data 2= 80X
Pooled data=155X
Will this strategy work?
1 answer
The short answer is yes, pooling the ~10Gbp runs will give you a total of ~20Gbp of raw sequence data. Keep in mind that after QC for insert length, read quality, adapter removal and removal of PCR duplicates you will be left with less usable data.
Log in to answer this question.