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Tandem Mass Tag (TMT) proteomics data analysis

Hi everybody,

I am approaching to analyse TMT proteomics data. For the subsequent data analysis, I was wondering about the use of an internal reference channel by pooling all the samples in the run. Is it required for a single MS run? What is the meaning of normalize by an internal pooled sample? If anyone has some interesting link which explains tmt-data analysis I would appreciate the advice!

Thank you, bye

tmt tandem-mass-tag proteomics
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updated by Ram Ram • written by F • F

1 answer

TMT technology can use up to 18 stable isotope tags, enabling the simultaneous comparison of protein levels across 18 different samples. TMT-based quantitative proteomics analysis technique can label virtually all peptide or protein samples, making it a widely adopted method for discovering quantitative protein biomarkers.

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written by TerryE.Chenier • TerryE.Chenier

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