How much coverage is needed for high-quality phylogenetic and admixture analyses using short-read WGS in plants?
Hi everyone, I am new at sequencing and bioinformatics. I’m planning to do Illumina short-read whole genome sequencing (WGS) on my plants. How much coverage do I need to get reliable results for phylogenetic and admixture analyses?
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Kevin Blighe
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How much short read coverage do other studies have which you want to replicate? Are those studies using whole genomes creating using long reads rather than the short reads you want ? Short read assemblies are incredibly fragmented for plants.