no "-" strand in transcriptome alignment
Hello everyone,
I am working with DRS reads and i have performed a transcriptome alignment of the DRS reads.
i can see that there are very few or no alignments on "-" strand and all are on "+" strand.
Am i missing some concept here? please anyone can explain?
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1 answer
This was answered in a separate thread: mapping to transcriptome.fasta vs genome.fasta
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