More posts like this
-
what should I do if I get a compromised sample of scRNA-seq data?
written by wangjb702 0Hi everyone, Recently, I got my snRNA-seq data. But after aligned to reference genome using cellranger, I got a wired barcode rank plot. what should …
-
Why my GWAS p-value QQ-plot is far above diagonal
written by Celia L. 0Hi. I'm trying to run GWAS pipeline using plink, but the results I got look really off. The QQ-plot of the p-values is far above …
-
Use limma for correlation analysis of RNA-seqdata and continous trait
written by Yibin 0Hi, there I want to use voom function in limma package to analyze the correlations between expression and age(continous data). I am not sure whether …
-
DESEQ2 analysis - PCA plot
written by shome 1![enter image description here][1]![enter image description here][2] I am not getting a lot of genes from my DESEQ2 analysis hence I was checking the PCA …
-
GSEA analysis
written by sarahawan92 1I ran the analysis in GSEA software and got the plots. But the plot doesn't look the way it has to be. I am posting …
-
strange QQ plots
written by dew 1Dear all, May I know if you have ever come across the QQ plot which does not display as close to a line, thank you …
-
deflated QQ plot but lambda >1
written by putty 4Dear All, What might be the reason for a deflated QQ-plot but lambda showing > 1 value. GWAS (case-control using glm-logistic regression adjusting for PC1-PC3 …
-
Can not plot degree distribution in Cytoscape Network Analyzer on logscale
written by bhodai 0I am using Cytoscape 3.9.0. When I tried - Tools > Analyze Network, the following window poped-up ![enter image description here][1] When I clicked on …
-
How to produce overlapping QQ-plot from GWAS results?
written by anikcropscience 28Hi, I would like to make an overlapping QQ-plot from the GWAS results. I have run two GWAS analyses and want to generate a figure …
-
[Errno 2] No such file or directory: '0-rawreads' when I run Falcon unzip with hifi data
written by Feng 0Hi all, I am running the falcon-unzip in a server, but got this error ![enter image description here][1] Here ia my fc_unzip.cfg file ![enter image …
Your "small bump" as you call it is not a big deal in a GWAS context, since it grossly corresponds to a unadjusted p-values = 0.01 and won't be considered after multiplicity correction. Another thing you can check is your p-value histogram. However if you want to remove this little jump above the straight line, depending on the model considered you can for example detect if there are additional sources of bias incorporating more covariables. Population structures in GWAS is often a big source of problems. You can edit your post with additional details on the model used, histogramm, etc for a more comprehensive answer.