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Measures For Assessing Quality Of The Dna Sample Before Sequencing?

What are good measures for assessing the quality of the DNA sample before sequencing with Illumina, Solid, 454, PacBio and Ion Torrent technology?
What are the protocols people use to assess the sample DNA is good before sequencing?

next-gen sequencing dna

1 answer

We typically run something along the lines of an Agilent Bioanalyzer and for cancer samples we run a CGH or SNP array to verify sample purity.

Though we do not do it routinely, I have heard of some groups running only small quantities of a library in a multiplexed lane (with several other test samples) to verify the integrity of the DNA. I would guess that this technique might be applicable to only a subset of possible sequencing technologies or applications.

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