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fmols for nanopore library loading

We use the LSK114 kit and in the protocol, when loading a library it asks for a specific amount of fmol which requires knowing the length of the DNA fragments. Is there a way to estimate this without having to analyse the sample? If not, is it better to over feed it?

nanopore sequencing libraryprep fmol lsk114
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updated by GenoMax GenoMax • written by njornet • njornet

1 answer

While this is not a bioinformatics question per se, you can use tapestation or a similar protocol to estimate fragment sizes.

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written by GenoMax GenoMax

Oh yeah, you're right I meant to ask in the nanopore community. My bad!

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written by njornet • njornet

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