I have a variant file (.vcf.gz), and I want to annotate this file using the Ensembl Rest API, particularly the Vep Rest API. I am new to this variant annotation; however, I have seen a couple of codes from the Ensembl page on how to do this, but I do not know where to input my data (sample.vcf.gz):

  import requests, sys

    server = "https://rest.ensembl.org"
    ext = "/vep/human/hgvs/9:g.22125504G>C?"

    r = requests.get(server+ext, headers={ "Content-Type" : "application/json"})

    if not r.ok:
      r.raise_for_status()
      sys.exit()

    decoded = r.json()
    print(repr(decoded))

I did try my hands on some codes as well but I always get this error message: Error: Failed to retrieve data from Ensembl REST API. Status code: 500.

    def annotate_variants_with_ensembl_rest_api(input_vcf, output_tsv):
    # Ensembl REST API URL for VEP
    server = "https://rest.ensembl.org/vep/human/hgvs"

    # Load compressed VCF file
    with gzip.open(input_vcf, 'rt') as vcf_file:
        vcf_content = vcf_file.read()

    # Define headers for the HTTP request
    headers = {
        'Content-Type': 'application/json',
        'Accept': 'application/json'
    }

    # Define data for the HTTP POST request
    data = {
        'hgvs_notations': vcf_content,
        'assembly_name': 'GRCh38',
        'plugin': ['human']
    }

    # Make an HTTP POST request to Ensembl REST API
    response = requests.post(server, headers=headers, json=data)

Any help will be much appreciated!