After merging batches, I got very segmented umap in scanpy
I merged four samples of 10X genomics and I did batch correction using harmony which converged after 4 iterations. I get this very segmented umap. But If I do each sample separately I get a normal clustering with (~28 clusters). What can be the reason?
Thank you in advance.
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MohammadAlkadi •
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Very sorry for the inconvenience. It turned out I merged the same batch 4 times.
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MohammadAlkadi •
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