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Using STAR aligner to build index of hg38

Hi, I'm using STAR aligner to build index, and I want to use hg38 as reference genome.

But I think hg38 is DNA level. Can it also be used as a reference genome for RNAseq? (My data is RNAseq)

hg38 star alignment rnaseq index

Have you gone through the manual here. In addition to the genome fasta file, you would also need a GTF file (of gene annotations) to build the STAR index.

Yes, I know also need a GTF file. So the GTF file will include RNA information? Thanks for your answer!!

GTF file includes gene models/annotation information.

You could also use a program like salmon/kallisto with just human transcriptome to do similar analysis.

But I am going to do variant calling later, so I think STAR is more appropriate. Thank you.

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