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Help with Error: None of the requested features were found: PECAM1 in slot data

Hi all, I decrease the resolution to get fewer clusters however I got the error when run:

FeaturePlot(object_combined, features = c('PECAM1'), min.cutoff = 'q10')
Error: None of the requested features were found: PECAM1 in slot data

Would you please have a suggestion? I don't have the error when keep high resolution. I appreciate it.

seurat multiome
• 3,851 views • link
written by Chris • Chris

1 answer

Can you check the available assays in your object_combined?

Assays(merged_seurat)
#it may show you as `predicted_ADT`, `RNA` etc. data slots
#your gene could in different assay, if it is not in predicted_ADT for example it could be in RNA data slot
DefaultAssay(merged_seurat) <- "RNA"
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updated by Ram Ram • written by bk11 • bk11

I think it is:

[1] "RNA"  "ATAC" "SCT"
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updated by Ram Ram • written by Chris • Chris

I would recommend you to check in each data slots.

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written by bk11 • bk11

When I run DefaultAssay(object_combined) <- "RNA", the error has gone. Thank you!

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written by Chris • Chris

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