Retrieve deseq2 normalised counts with only results file available
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9 months ago
BM ▴ 70

I have several Deseq2 results file, so a csv file with ensembl name, baseMean , log2FoldChange, lfcSE, stat, pvalue, padj columns. Unfortunately, I don't have the original count files.

Is there a way to extract Deseq2 Normalised Counts from the results file only? Is this possible?

Thanks in advance

RNAseq Deseq2 Normalised Counts • 557 views
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Entering edit mode
9 months ago
ATpoint 82k

No, this information is lost.

Note that without counts and raw data at hand your analysis is not reproducible, so treat results with care. I would personally not even use the results productively other than for internal hypothesis generation since you can never rerun analysis.

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9 months ago
Ayeh • 0

unfortunately, No, its not possible. if you have fastq files or every intermediate files before construction of count matrix, you can do all thing at first to great your count matrix again.

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