Estimating sample size from bulk RNAseq data
Is it possible to estimate the sample size (number of cells) in a bulk RNASeq sample data?
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ahmad mousavi
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You may find an estimation of cellular type composition, with deconvolution approaches. It's barely impossible to assess the absolute number of all the cells in your bulk.
Its hard to say how many cells was used in a Bulk RNA-SEQ, because we ignore the cells identity and extract RNA from whole cells.
Maybe you can try a control gene in the sample as housekeeping and count the number isoform/gene seen in the sample as the cell numbers, but its hard.