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clustering changes in complex heatmap everytime

Hi all, I created a heatmap with using row_km option. And I am trying to get rownames in the clusters with row_order() option but everytime I run it gives different heatmap and clustering. Is there any way to do this stable and get one heatmap with one type of clustering? set.seed(123) is set

enter image description here

rnaseq heatmap complex
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written by biology_inform • biology_inform

This is strange to me. Just out of curiosity, did you use set.seed(123) right before using Heatmap(..., row_km = ..., column_km = ...)?

BTW, your heatmap looks nice (aesthetically) ;)

Regards,

Nitin N.

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written by Nitin Narwade Nitin Narwade

firstly, thanks :) I used set.seed(123) just before raw data import which is in the beginning before the data ordering. Should I use just before Heatmap(....)?

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written by biology_inform • biology_inform

1 answer

Hi, this exact github issue answers your question https://github.com/jokergoo/ComplexHeatmap/issues/737

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written by Ming Tommy Tang Ming Tommy Tang

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