Hi, I've been running Kraken2 and KrakenUniq (using the --report-minimizer-data flag) on my metagenomic samples. When I sum up the per level read counts for …
Hi all, I have an RNA-seq dataset (comparing two different conditions) that I have analyzed using four different methods of differential expression (DE) analysis: DESeq2, …
Hi, I often read in articles that read count RNASeq expression matrices (rows=genes / columns=samples) have been fpkm-normalized, median-centered, log2 transformed. In which order these …
Hi everyone, I conducted RT-qPCR to validate my transcriptome analysis. In my transcriptome data, the genes are expressed in Stage 1, but are not expressed …
Hi all, I'm analysing some microarray data and just calculated p-values using a t-test between 2 conditions (each with 3 replicates; total number of genes …
Can you upload a snapshot or provide some output?
Regards,
Nitin N.